XRCC4, is a protein of unknown function. The Ku70 protein is an additional component of DNA-PK; Ku70 forms a het-erodimer with Ku80 to generate the DNA end-binding com-ponent of the enzyme. To test putative functions for Ku70, we have used gene-targeted mutation to generate a murine em-bryonic stem cell line which lacks Ku70 expression. We find

av K Söderlund Leifler · 2009 — associated with cancer due to gain-of-function mutations are called proto- oncogenes strand break by the end-binding heterodimer of the Ku70 and Ku80 pro-.

Mutations in any of the three subunits of this protein kinase (Ku70, Ku80 and DNA-PKcs) lead to sensitivity to ionizing radiation (IR) and to DNA double-strand breaks, and V(D)J recombination product formation defects. Ku is thought to function as a molecular scaffold to which other proteins involved in NHEJ can bind, orienting the double-strand break for ligation. The Ku70 and Ku80 proteins consist of three structural domains. The N-terminal domain is an alpha/beta domain. This domain only makes a small contribution to the dimer interface. Sequence and domain structure of the zebrafish Ku80 gene.

Ku70 and ku80 function

Together, Ku70 and Ku80 make up the Ku heterodimer, which binds to DNA double-strand break ends and is required for the non-homologous end joining (NHEJ) pathway of DNA repair. It is also required for V(D)J recombination, which utilizes the NHEJ pathway to promote antigen diversity in the mammalian immune system. Recognition of DNA double‐strand breaks during non‐homologous end joining is carried out by the Ku70–Ku80 protein, a 150 kDa heterodimer that recruits the DNA repair kinase DNA‐dependent protein kinase catalytic subunit (DNA‐PKcs) to the lesion. The atomic structure of a truncated Ku70–Ku80 was determined; however, the subunit‐specific carboxy‐terminal domain of Ku80—essential for binding to DNA‐PKcs—was determined only in isolation, and the C‐terminal domain of Ku70 Ku70 and Ku80 in A. thaliana.

Structural Basis of Importin-α-Mediated Nuclear Transport for Ku70 and Ku80 Agnes A. S. Takeda1, Andrea C. de Barros1, Chiung-Wen Chang2, Boštjan Kobe2 and Marcos R. M. Fontes1⁎ 1Departamento de Física e Biofísica, Instituto de Biociências, Universidade Estadual Paulista, Botucatu, SP 18618-970, Brazil 2School of Chemistry and Molecular Biosciences, Institute for Molecular Bioscience

Ku70‐knockout mice, in contrast, appear to be tumor prone and develop thymic lymphomas with high incidence (30, 36). Finally, in agreement with a Ku70‐specific role in life‐span regulation, it was recently shown that knockdown of Ku70, but not Ku80, significantly extended life span in the p53‐null background .

26 Dec 2018 The Ku heterodimer, composed of Ku70 and Ku80, is best characterized for its role in repairing double-stranded DNA breaks but is also known

Ku80 works as a heterodimer with Ku70. However, it is not yet clear whether KARP-1 forms a heterodimer with Ku70 and works as a heterodimer. factors, including Ku70, Ku80, XRCC4, and Lig4 (Taccioli et al., 1994; Li et al., 1995; Gu et al., 1997; Frank et al., 1998). Ku70 and Ku80 form the “Ku” DNA end-binding complex which functions as the DSB recognition com-ponent of C-NHEJ, whereas the XRCC4/ Lig4 complex is specific for C-NHEJ ligation. (2002) Korabiowska et al.

Our previous study with Ku70−/− and Ku80−/− mice, and cell lines has shown that Ku70- and Ku80-deficiency compromises the ability of cells to repair DNA double-strand breaks, increases radiosensitivity of cells, and Binds to naturally occurring chromosomal ends, and therefore provides chromosomal end protection. Required also for telomere recombination to repair telomeric ends in the absence of telomerase.
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I made another example for one of my sensors. Now we are using the KY-018 Photoresistor Sensor as a switch. Find this and comprised of two subunits, Ku70 and Ku80, that is best characterized for its central role as the initial DNA end binding factor in the ''classical'' non- homologous 26 Dec 2018 The Ku heterodimer, composed of Ku70 and Ku80, is best characterized for its role in repairing double-stranded DNA breaks but is also known Furthermore, it has been reported that the Ku70 subunit contains the ATPase activity and is able to perform helicase function independently of Ku80 (Ochem et 25 May 2007 Summary: Subunit of the Ku70:Ku80 complex; binds RNA and damaged Composed in yeast of Yku70p and Yku80p, Ku functions in genome 17 Jul 2019 We also discuss its lesser-known functions, the pharmacotherapies Ku70 and Ku80 contain three domains: an alpha helix/beta barrel von av K Söderlund Leifler · 2009 — associated with cancer due to gain-of-function mutations are called proto- oncogenes strand break by the end-binding heterodimer of the Ku70 and Ku80 pro-. av P Håkansson · 2006 — regarding the function of an alternative mammalian RNR small subunit, and on the role of is the binding of the end-binding Ku70/Ku80 complex, leading to the. Pia C. Maly Sundgren, 2017 Sep 12, Neuroimaging: Anatomy Meets Function.

We find Its function remains elusive. We reisolated CLUyXIP8 by yeast two-hybrid analyses using as bait the DNA double-strand break repair protein Ku70.
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6 Oct 2020 Knockdown of Ku70 or Ku80 in naïve mice elicited mitochondrial collapse or ER stress, leading to bronchial epithelial cell apoptosis and

Ku is thought to function as a molecular scaffold to which other proteins involved in NHEJ can bind, orienting the double-strand break for ligation. The Ku70 and Ku80 proteins consist of three structural domains. The N-terminal domain is an alpha/beta domain. This domain only makes a small contribution to the dimer interface. Ku70 is an evolutionarily conserved protein that has functions in DNA repair and maintenance [96]. It is a heterodimeric protein made up of Ku70 and Ku80 [97] . We (unpublished data) and others [43] had evaluated and shown that proteins involved in DNA repair including DNA-PK, p53, ATM and Ku70 had no effect on the type-I-IFN response induced by cytosolic dsDNA stimulation.

Ku70 and Ku80. Ku70 and Ku80 form a heterodimer, Ku, which possesses DNA end-binding activity (Mimori and Hardin, 1986 ). Purified Ku protein was found to promote the association of two DNA molecules in vitro; thus, it was proposed to possess end bridging or alignment activity ( …

Ku70 and Ku80 form a heterodimer, Ku, which possesses DNA end-binding activity (Mimori and Hardin, 1986 ). Purified Ku protein was found to promote the association of two DNA molecules in vitro; thus, it was proposed to possess end bridging or alignment activity ( … We conclude that Ku70 and Ku80 may have functions in V (D)J recombination and DNA repair that are independent of DNA-PKcs.